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| Registros recuperados: 13 | |
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| Vonau, Vincent; Matehau, Ariora; Rouxel, Catherine; Cochard, Jean-claude. |
| La congélation du sperme et des embryons d'huîtres perlières est un moyen de renforcer la sécurité du potentiel économique que représente la Perliculture en Polynésie Française. Elle permet la conservation, à faible coût, de la biodiversité ex-situ des populations d'huîtres perlières sauvages et des génotypes de sujets présentant un intérêt particulier pour l'industrie de la perle. Un avantage complémentaire de la cryoconservation sera de faciliter l'avancement des programmes d'amélioration génétique. À ce jour, les divers travaux réalisés sur les gamètes des mollusques bivalves ont permis de congeler la laitance de différentes espèces autres que Pinctada margaritifera. Cependant, quelle que soit l'espèce, le protocole complet n'a pas été validé pour... |
| Tipo: Text |
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| Ano: 2005 |
URL: http://archimer.ifremer.fr/doc/00304/41501/40709.pdf |
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| Le Moullac, Gilles; Hui, Belinda; Vonau, Vincent; Levy, Peva; Cochard, Jean-claude. |
| Preliminary experiments allowed defining basic requirements of Pinctada margaritifera for somatic and gonadic growth in controlled conditions. This study was continued by observation of the gametogenesis at a cellular level. In April/May 2008, 125 oysters were conditioned for 30 days in 25 tubular tanks (30 L). Water was renewed 4 times/hour. A mixed (v:v) diet of Isochrysis galbana (T-Iso) and Chaetoceros gracilis was supplied continuously. A control batch of 125 oysters was maintained in trays in the lagoon. An initial sample of 30 individuals was made. Gonadic changes were first characterized using a gonadal development index (GDI) based on the ratio of the gonad area to the total visceral mass area on a sagittal section of the body. Histological... |
| Tipo: Text |
Palavras-chave: Pearl oyster; Reproduction; Gametogenesis; Broodstock. |
| Ano: 2009 |
URL: http://archimer.ifremer.fr/doc/00029/14010/11196.pdf |
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| Le Moullac, Gilles; Soyez, Claude; Sham-koua, Manaarii; Levy, Peva; Moriceau, Jacques; Vonau, Vincent; Maihota, Mayalen; Cochard, Jean-claude. |
| This study aimed to model the food intake of P. margaritifera in order to examine the relationship between food level and reproductive activity. The effect of microalgae concentration on ingestion rate and assimilation efficiency was studied over a broad concentration range, using a mixture of Isochrysis galbana and Chaetoceros gracilis. Reproductive effort was assessed using three microalgae concentrations of 0.5, 7 and 18 cell μL-1. Reproductive status was assessed by gonad development index (GDI) – the ratio of the gonad surface to the visceral mass surface – and histological analysis of the gonad based on the presence (continuous or discontinuous) or the absence of gonial cells (GC). Ingestion is a saturating function of seston concentration for... |
| Tipo: Text |
Palavras-chave: Pinctada margaritifera; Food level; Ingestion rate; Reproduction; Gametogenesis. |
| Ano: 2013 |
URL: http://archimer.ifremer.fr/doc/00050/16085/13562.pdf |
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| Hui, Belinda; Demoy-schneider, Marina; Vonau, Vincent; Le Moullac, Gilles; Moriceau, Jacques; Le Pennec, Marcel; Cochard, Jean-claude. |
| Cryopreservation is a useful tool for genetic improvement which has been applied to several bivalve mollusk species. It would allow to keep the gametes of selected individuals and to preserve ex-situ the biodiversity of wild populations of the black-lip pearl oyster Pinctada margaritifera threatened of standardization by the seed transfers between the islands in French Polynesia. The ability to cryopreserve spermatozoa would bring about significant benefits to the cultured black pearl industry. Sperm freezing supposes the control of different steps: preparation of breeders, sperm collection, evaluation of sperm quality and the freezing process itself. Broodstock conditioning in hatchery allows to obtain gametes from the pearl oyster. Sperm quality is... |
| Tipo: Text |
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| Ano: 2009 |
URL: http://archimer.ifremer.fr/doc/00029/14043/11238.pdf |
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| Arnaud, Sophie; Vonau, Vincent; Rouxel, Catherine; Bonhomme, Francois; Prou, Jean; Goyard, Emmanuel; Boudry, Pierre. |
| In order to study further the genetic structure of the pearl oyster Pinctada margaritifera in French Polynesia with a special consideration for the sampling scale, we analyzed or re-analyzed sets of data based on nuclear DNA markers obtained at different spatial scales. At a large scale (several 1,000 km), the remote Marquesas Islands were confirmed to be significantly differentiated from Tuamotu-Gambier and Society archipelagos, with a marked difference however for the two main islands that are different from each other. At a medium scale (several 10 to several 100 km), overall homogeneity was observed within and between these two archipelagos, with some exceptions. This could be attributed both to large-scale larval dispersal and to human-driven spat... |
| Tipo: Text |
Palavras-chave: Population genetics; Polynesia; Bivalve; Pearl oyster; Benthic organisms; Sampling strategy; Genetic patchiness. |
| Ano: 2008 |
URL: http://archimer.ifremer.fr/doc/2008/publication-4960.pdf |
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| Vonau, Vincent; Rouxel, Catherine; Saulnier, Denis; Cochennec-laureau, Nathalie; Nedelec, Georges; Goyard, Emmanuel. |
| The tropical seabass Lates calcarifer was introduced in Tahiti in 1984 and then was domesticated without other introduction. Three successive generations have been obtained in captivity. Tissue samples of the 38 animaIs which represent the total Tahitian broodstock were preserved in alcohol to be genotyped with four microsatellite markers (Yue et al., 2001). Three of four described markers were successfully transferred to the laboratory of genetics of Tahiti (marker LcaM01, LcaM02, LcaM03), as the revelation technology available locally did not allow the use of LcaM04 in routine. The results show that the genetic diversity of the tahitian broodstock is not equivalent of the one of the species, which could be explained by the small size of the founder... |
| Tipo: Text |
Palavras-chave: Lates calcarifer; Marqueurs microsatellites; Reproducteurs; Variabilité génétique; Lates calcarifer; Microsatellite markers; Breeding stock; Genetic diversity. |
| Ano: 2003 |
URL: http://archimer.ifremer.fr/doc/00142/25300/23372.pdf |
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| Arnaud, Sophie; Goyard, Emmanuel; Prou, Jean; Vonau, Vincent; Bonhomme, Francois; Boudry, Pierre. |
| Exploitation of the pearl oyster Pinetada margaritifera, which has developed in French Polynesia over the last twenty years, is based mainly on the collection of natural spat. Recruitment is highly variable in space and time and so spat is very often transferred by the farmers between atolls or even between archipelagos. In previous studies, anonymous nuclear markers demonstrated that This development has coincided with a genetic homogenisation of the wild stocks since the 1980s. This suggests a high level of reproductive success on farms, which is likely to be due to the high density of animaIs when compared to the density in the wild. We used the same markers to show that the level of genetic variability observed on farms is not significantly different... |
| Tipo: Text |
Palavras-chave: French Polynesia; Genetic diversity; Spat; Pinctada margaritifera; Pearl oyster; Polynésie française; Diversité génétique; Naissain; Pinctada margaritifera; Huître perlière. |
| Ano: 2005 |
URL: http://archimer.ifremer.fr/doc/2005/publication-3822.PDF |
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| Hui, Belinda; Vonau, Vincent; Moriceau, Jacques; Tetumu, Roger; Vanaa, Vincent; Demoy-schneider, Marina; Suquet, Marc; Le Moullac, Gilles. |
| Cryopreservation is a valuable tool for genetic improvement programs. Several bivalve mollusc species have already been the subject of such programs and the Tahitian black pearl oyster industry is now planning the development of selective breeding for desirable traits in Pinctada margaritifera. The ability to cryopreserve spermatozoa would, therefore, offer significant benefits to the cultured black pearl industry. Spermatozoa were cryopreserved with CPA 0.7 M trehalose in 0.8 M Me2SO and a two-step freezing process was used: straws were first maintained in nitrogen vapour for 10 minutes, then directly plunged into liquid nitrogen and stored for one week before use. The viability of thawed sperm was 23 % lower than that of fresh sperm. When using thawed... |
| Tipo: Text |
Palavras-chave: Cryopreservation; Spermatozoa; Fertility; Larval rearing; Pearl oyster; Pinctada margaritifera. |
| Ano: 2011 |
URL: http://archimer.ifremer.fr/doc/00040/15098/12446.pdf |
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| Le Moullac, Gilles; Rouxel, Catherine; Vonau, Vincent; Ledu, Christophe; Cochard, Jean-claude. |
| Afin de mener à bien le programme Maîtrise de la Reproduction chez l’huître perlière Pinctada margaritifera dont la perspective est l’amélioration génétique, il est essentiel de contrôler les croisements au niveau individuel. Ces croisements ne pourront être contrôlés que dans la mesure où les gamètes mâles et femelles sont obtenus séparément. Ceci permettra aussi de valider les travaux en cryoconservation des spermatozoïdes et de faciliter la production de polyploïdes. Ces travaux répondent aussi aux préoccupations d’amélioration génétique. Cette étude est basée sur deux approches complémentaires. D’une part, l’étude de la maturation ovocytaire in vitro afin de déterminer les conditions qui permettront de disposer d’ovocytes prêts à être fécondés. D’autre... |
| Tipo: Text |
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| Ano: 2003 |
URL: http://archimer.ifremer.fr/doc/00182/29290/27731.pdf |
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| Bierne, Nicolas; Bezuart, Ivan; Vonau, Vincent; Bonhomme, François; Bedier, Edouard. |
| Correlation between DNA microsatellite heterozygosity and growth rate was investigated in two hatchery-propagated stocks of the shrimp Penaeus stylirostris, which had been genetically isolated from wild founders for 17 generations, and from each other for five more generations. presumed demographic history of these populations suggested that they were maintained at small effective population sizes. and this was confirmed by significant changes in allelic frequencies between the two stocks. Despite a small sample size (n = 60) and a small number of loci screened, a significant positive correlation was detected between microsatellite tri-locus heterozygosity and growth rate in one of the two stocks (r = 0.3, P = 0.02). In the other stock (n = 48), the... |
| Tipo: Text |
Palavras-chave: Penaeus stylirostris; Heterozygosity; Marker associated heterosis; Inbreeding depression; Microsatellites. |
| Ano: 2000 |
URL: http://archimer.ifremer.fr/doc/2000/publication-753.pdf |
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| Arnaud, Sophie; Goyard, Emmanuel; Vonau, Vincent; Herbaut, C; Prou, Jean; Saulnier, Denis. |
| Most bivalves species of the genus Pinctada are well known throughout the world for production of white or black pearls of high commercial value. For cultured pearl production, a mantle allograft from a donor is implanted into the gonad of a recipient oyster, together with a small inorganic bead. Because of the dedifferentiation of cells during the first steps of the host oyster's immunological reaction, so far the fate of the graft and its exact role in the process of pearl formation could not be determined via classical histological methods. Here we report the first molecular evidence of the resilience of the graft in the recipient organism by showing that cells containing genome from the donor are still present at the end of pearl formation. These... |
| Tipo: Text |
Palavras-chave: Genotype; Pearl; Pearl sac; Grafting; Biomineralization; Pearl oyster. |
| Ano: 2007 |
URL: http://archimer.ifremer.fr/doc/2007/publication-2456.pdf |
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| Goyard, Emmanuel; Arnaud, Sophie; Vonau, Vincent; Bishoff, Vincent; Mouchel, Olivier; Pham, Dominique; Wyban, Jim; Boudry, Pierre. |
| The Latin American shrimp Litopenaeus stylirostris was introduced in three different Pacific islands (Tahiti, New Caledonia via Tahiti, and Hawaii) and hatchery-propagated for 7-25 generations to develop shrimp farming based on these domesticated stocks. Three microsatellite markers have been used in an attempt to assess the genetic bases of the populations available to start a selective breeding program. The comparison of eight hatchery stocks (five New Caledonian, two Hawaiian and one Tahitian stocks) and one wild Ecuadorian population showed a much lower variability in the domesticated stocks than in the wild population, especially in New Caledonia and Tahiti (2-3.7 vs. 14-27 alleles per locus; 20-60% vs. 90% expected heterozygosity). The Tahitian and... |
| Tipo: Text |
Palavras-chave: Microsatellite marker; Genetic variability; Domestication; Litopenaeus stylirostris; Shrimp. |
| Ano: 2003 |
URL: http://archimer.ifremer.fr/doc/2003/publication-596.pdf |
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| Registros recuperados: 13 | |
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